这竟然是楼里目前最高赞的楼层，不知道说什么好😂
zhengxumaomao 发表于 2021-06-12 04:08
我的天， 并不是所有的MD都是PHD的。有了PHD说明这些人希望在偏基础研究上写文章。类比的话就是，PHD会有更多老鼠，斑马鱼，果蝇的实验， MS会更多偏向clinic的数据。
如果一个人是MD & PHD，我们都玩笑，他的最大长处是，他可以跟PHD说MD相关的事情，跟MD说PHD的事情。 还有很多人可能不知道，如果有一篇PNAS基本可以在美国藤校申请天牛的。我家LD上个老板就是凭一篇PNAS去了斯坦福，还带天牛。
还有，你不懂不确信，其实可以查查他的citation，他应该不是那张每年刷文章的，但也不低。
回复 165楼cafe1123的帖子自己吹的， 还有人信。
cactus_mei 发表于 2021-06-12 07:05
确实是他最早提出RNA技术做疫苗的
In 1989, Robert W. Malone, P. Felgner, et. al. developed a high-efficiency in-vitro and in-vivo RNA transfection system using cationic liposomes, which were used "to directly introduce RNA into whole tissues and embryos", as well as various cells types. The term and idea of "RNA as a drug" is first described in this paper. [15] Then, in 1990, Jon A. Wolff, Robert W Malone, et. al. demonstrated the idea of nucleic acid-encoded drugs by direct injecting in vitro transcribed (IVT) mRNA or plasmid DNA (pDNA) into the skeletal muscle of mice which expressed the encoded protein in the injected muscle. These studies were the first evidence that in vitro transcribed (IVT) mRNA could deliver the genetic information to produce proteins within living cell tissue. [16][17]
The first mRNA vaccine experiments were carried out by P. Felgner, J. Wolff, G. Rhodes, R.W. Malone and D. Carson. P. They completed a number of mRNA vaccination studies that resulted in nine patents on mRNA vaccination with a shared priority date of March 21, 1989. One experiment documented that NEF (an HIV protein) mRNA vaccination in mice, followed by HIV challenge reduced positively stained cells by 2-fold and p24 expression was reduced by 50% at eight weeks. [18][19][20]
